| Tropical Andes | 1988 | Recognized for the highest richness of plant and vertebrate species, this hotspot is diverse due to its altitude variation and climate. |
| Tumbes-Chocó-Magdalena | 1990 | Situated along the Pacific coast of South America, it’s noted for its tremendous floral diversity. |
| Sundaland | 1992 | Located in Southeast Asia, Sundaland includes the western half of the Indo-Malayan archipelago and is home to significant mammalian, bird, and plant diversity. |
| Mediterranean Basin | 1996 | Characterized by its unique and rich plant biodiversity. It harbors approximately 25,000 plant species, 60% of which are unique to this region. |
| California Floristic Province | 1996 | Known for its variety of endemic and threatened species, particularly related to plant life. |
| Madagascar and the Indian Ocean Islands | 2000 | Known for its incredibly high endemism level and unique species, such as various types of lemurs. |
| Eastern Afromontane | 2003 | Stretches across a series of highlands in eastern Africa, it’s home to a wealth of unique, endemic species. |
| Guinean Forests of West Africa | 2006 | Covers a series of highlands and forests, with a high level of mammalian and bird diversity. |
| New Caledonia | 2008 | Known for its high percentage of unique plant diversity. |
| Himalaya | 2011 | Home to significant diversity, largely due to its varied climates and high-altitude environment. |
